Journal: Optics and Lasers in Engineering
Article Title: Hydrothermal treatment of laser micro-textured Ti6Al4V systems for osseointegration of orthopaedic implants
doi: 10.1016/j.optlaseng.2024.108511
Figure Lengend Snippet: Fig. 1. 3D (left) and 2D (middle) optical profilometry images of the GW60, GW90 and GW120 micro-pillar textured samples and fluorescence images of MSC culture (right). Fluorescence images of MSC correspond to the merge of three channels: Phalloidin staining (green fluorescence) highlighting the cytoskeleton of the cells, DAPI staining (blue fluorescence) demonstrating the nuclei of cells, and RUNX2 expression detected with brown fluorescence staining. All fluorescence images include a 100 μm scale bar.
Article Snippet: Then, cells were blocked with 3 % BSA in PBS for 1 h at room temperature and incubated at 4 ◦C overnight with rat anti-human antibody specific for RUNX2 (232902; R&D Systems, USA).
Techniques: Fluorescence, Staining, Expressing